Modulation of the host cell RNA splicing program by the gastric pathogen Helicobacter pylori

Authors

  • Frithjof Glowinski Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin
  • Fernando Garcia-Alcalde Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin
  • Konstantin Okonechnikov Department Molecular Biology, Max Planck Institute for Infection Biology, Berlin
  • Thomas F Meyer Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin

DOI:

https://doi.org/10.14806/ej.19.A.652

Abstract

Helicobacter pylori is a Gram-negative bacterial pathogen colonizing the human stomach. Infection with H. pylori  causes chronic inflammation of the gastric mucosa and may lead to peptic ulceration and/or gastric cancer. Using a quantitative phosphoproteomic approach several splicing factors were found to be differentially phosphorylated upon infection (Holland et al. 2011).Functional consequences of the differential phosphorylation were analyzed using RNA-seq. Analysis revealed differntially spliced genes in the infected cells. Analysis was performed using either DEXSeq or Cufflinks. Results of this tools will be compared to an in-house approach for detection of genes with significantly altered isoform expression.

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Published

2013-04-08

Issue

Vol. 19: Supplement A

Section

Posters

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