Power and limitations of RNA-Seq: findings from the SEQC (MAQC-III) Consortium

Authors

  • Pawe? Piotr ?abaj Chair of Bioinformatics Research Group, Boku University Vienna

DOI:

https://doi.org/10.14806/ej.21.A.831

Abstract

We present a multi-centre cross-platform study of the US-FDA MAQC/SEQC-consortium, introducing a landmark RNA-Seq reference dataset comprising 30 billion reads. The study design features known mixtures, high-dynamic range ERCC-spikes, and a nested replication structure which are critical for the development and validation of novel or improved algorithms and data processing pipelines, as none of the examined technologies can provide a ‘gold standard’.
EJ 21.A

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Published

2015-03-25

Issue

Vol. 21: Supplement A

Section

Keynote Lectures

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