A comprehensive comparison between reference-based and ‘de novo’ isoform assembly approaches
DOI:
https://doi.org/10.14806/ej.19.A.621Keywords:
RNA-seq, de novo assembly, reference-based assemblyAbstract
The reconstruction of the transcriptome can be performed following two different approaches, a reference-based method in which reads are mapped back to a reference genome, and a ‘de novo’ assembly strategy where reads are compared to each other to reconstruct expressed isoforms without the need of using a reference genome. In the present studio we provide a comprehensive comparison between these two transcriptome analysis methodologies for isoforms reconstruction based on genome annotation and isoform expression levels using a Human sample
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2013-04-08
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